LOCUS       40924_23639       11441 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB685 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Gateway Binary Vectors with Organelle-Targeted Fluorescent Proteins 
            for Highly Sensitive Reporter Assay in Gene Expression Analysis of 
            Plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11441)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane 
            University, Interdisciplinary Center for Science Research; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11441)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11441)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane University, 
            Interdisciplinary Center for Science Research; 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2071..2091
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             2092..2802
                     /codon_start=1
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      2827..3079
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3113..3129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3137..3153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3161..3191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3206..3227)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3263..3515)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3678..4226)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4278..4457)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4963..4987)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5508..6296
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6545..7133
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7319..7459)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7803..7997)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8066..9136)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9568..10194)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatggct ccaaagaaga agagaaaggt catggtgtct
     2101 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     2161 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     2221 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     2281 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc
     2341 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     2401 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     2461 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     2521 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
     2581 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
     2641 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg
     2701 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     2761 agatactgcg acctccctag caaactgggg cacaaactta attgagctta gagctcgaat
     2821 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     2881 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     2941 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     3001 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     3061 tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc
     3121 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3181 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc
     3241 gcgcctattt ctattaattc ccgatctagt aacatagatg acaccgcgcg cgataattta
     3301 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     3361 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     3421 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     3481 cttaagaaac tttattgcca aatgtttgaa cgatcgggga aattcgagct ggggacgtcc
     3541 ccagctcggt accccctcct gggcacggtc gtacgcgatc cgccgcggtt ccacctcacc
     3601 attgcgggta tgccagttga gttcgatgag ttcccgtacc agctccaact cgggcgatgc
     3661 cacgcgcact ccgttcagat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
     3721 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
     3781 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
     3841 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
     3901 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
     3961 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
     4021 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
     4081 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
     4141 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
     4201 tcggccgggc gtcgttctgg gctcatatct tattgccccc cgggatcaga tctggattga
     4261 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
     4321 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
     4381 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc
     4441 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
     4501 ggggtcataa cgtgactccc ttaattctcc gctcatgatc ttgatcccct gcgccatcag
     4561 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
     4621 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
     4681 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
     4741 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc
     4801 tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgaagct
     4861 gatccgtcga gattaataga aataaattca gcctaattcg gcgttaattc agtacattaa
     4921 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     4981 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     5041 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     5101 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     5161 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     5221 gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     5281 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     5341 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     5401 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     5461 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg
     5521 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     5581 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     5641 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     5701 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     5761 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     5821 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     5881 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     5941 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     6001 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     6061 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     6121 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     6181 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     6241 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
     6301 gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt
     6361 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     6421 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     6481 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6541 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6601 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6661 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     6721 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6781 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6841 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6901 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6961 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7021 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7081 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7141 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7201 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7261 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7321 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     7381 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     7441 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     7501 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     7561 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     7621 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     7681 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     7741 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     7801 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     7861 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     7921 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     7981 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     8041 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     8101 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     8161 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     8221 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     8281 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     8341 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     8401 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     8461 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     8521 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     8581 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     8641 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     8701 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     8761 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     8821 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     8881 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     8941 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     9001 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     9061 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     9121 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
     9181 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
     9241 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
     9301 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
     9361 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
     9421 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
     9481 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
     9541 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
     9601 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
     9661 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
     9721 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
     9781 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
     9841 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
     9901 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
     9961 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
    10021 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
    10081 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
    10141 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
    10201 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
    10261 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
    10321 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
    10381 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
    10441 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
    10501 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
    10561 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
    10621 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
    10681 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
    10741 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
    10801 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
    10861 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
    10921 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
    10981 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
    11041 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
    11101 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
    11161 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
    11221 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
    11281 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
    11341 cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct
    11401 gtggttggca tgcacataca aatggacgaa cggataaacc t
//
